Infectious bovine rhinotracheitis

Description

Infectious bovine rhinotracheitis is a highly contagious respiratory disease, most commonly in feedlot cattle and affecting both young and older cattle. The viral infection alone causes only mild to moderate respiratory signs, but it predisposes to secondary bacterial pneumonia which can cause death.

Other syndromes observed include infectious pustular vulvovaginitis, balanoposthitis, conjunctivitis and encephalomyelitis (very rare).

Diagnosis and tests available

Diagnosis

Diagnosis is strongly supported by history, clinical signs, histopathology and serology tests and confirmed by PCR.

Tests available

Click on + in the tables below for sampling requirements and additional test information

Code	Name	Samples required	Species	Turnaround time	Price (excl. GST)	Notes	Test days
HIST_HE1	Histopathology – Preparation & Examination	Fixed Tissues- General Fresh tissues immediately placed in formalin Tissue pieces should not be greater than 1cm cubed to ensure proper fixation Submit fixed in neutral buffered formalin at a 10:1 ratio of formalin: tissue Fixed Tissues- Nervous tissues Submission of whole brain is required to ensure preservation of TSE target sites submit fixed in neutral buffered formalin at a ratio of 10:1 formalin: tissue Brains submitted for Nervous tissues are processed once a week, separate to general tissues to ensure consistent and reliable results. Urgent cases can be processed out of cycle if clearly marked on the Submission Form however this may reduce the quality of the brain histology results, so should only be requested in extremely urgent cases. Fresh Tissues Fresh tissues can be submitted following necropsy The quality of the tissues will be reduced due to the effects of autolysis so ideally fixed tissue should be submitted in neutral buffered formalin at a ratio of 10:1 formalin: tissue	All species	Up to 10D	$84.95	D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.	Monday - Friday
IBR_ABEL	Infectious Bovine Rhinotracheitis (IBR) ELISA	Clotted blood (Red top) 10ml clotted blood collected in a red top tube Submit chilled	Bovine	Up to 7D	$21.80	D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.	Weekly
IBR_RT_PCR	Infectious Bovine Rhinotracheitis (IBR) Real–time PCR	Fresh tissue Fresh lung collected at post mortem Submit chilled Swab in PBGS Nasal or genital swab from acutely affected animals Send in viral transport media (PBGS) Semen Semen straws or raw semen Please contact Customer Service prior to submission. For regulatory testing samples must be shipped at specified temperatures)	Bovine	2-3D	$109.35	D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.	According to demand
IBR2_VI or IBR3_VI	Infectious Bovine Rhinotracheitis Virus Isolation (2 or 3 passes)	Semen Semen straws or raw semen Please contact Customer Service prior to submission. For regulatory testing samples must be shipped at specified temperatures)	Bovine	3-4W	$174.10	D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.	According to demand
IBR_VN	Infectious Bovine Rhinotracheitis (IBR) Virus Neutralisation test (VNT)	Clotted blood (Red top) 10ml clotted blood collected in a red top tube Submit chilled	Bovine	1-2W	$55.15	D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.	Batch tested on Thursday

Additional Sample requirement Information:

Histopathology sample requirements
Fixed Tissues Lung collected at post mortem and submit fixed in neutral buffered formalin at a 10:1 ratio of formalin: tissue

Histopathology sample requirements

Fixed Tissues

Lung collected at post mortem and submit fixed in neutral buffered formalin at a 10:1 ratio of formalin: tissue

Additional Information

Customer Service

Contact Customer Service for enquiries relating to testing and results, quotations and pricing, couriers, sample submission and invoicing.

NSW DPIRD's laboratories are committed to continual improvement of services. If you would like to provide feedback, please fill in and submit the online feedback form.

CONTACT DETAILS
Phone	1800 675 623
Email	laboratory.services@dpird.nsw.gov.au
Operating Hours	8:30am-4:30pm Monday-Friday (excluding public holidays) 8:30am-12:00pm Saturday (for deliveries only)
Postal Address	Private Bag 4008, Narellan NSW 2567

Quotations

For Animal export, Plant health or Veterinary quotations, please complete the online quotation request

Submission Forms

Veterinary Specimen Advice Form

Please Note: These documents are not fully web accessible, please contact Customer Service (laboratory.services@dpird.nsw.gov.au) for more information.

Courier services and specimen delivery

For NSW submissions, please contact the relevant Customer Service team for courier account details and to order consignment notes.

For submitters outside of NSW, Couriers can be contacted directly to make bookings and arrange collection of packages at the submitters own cost.

Commercial couriers may use either road or air transport and specimens should therefore be packed in accordance with International Air Transport Association (IATA) requirements. Most submission sent by veterinarians to the APHL for testing are defined as “Biological Substance Category B” and must be packed according to the IATA packing instructions 650 (Biological Substance Category B). As requirements for transport can change, customers should consult with their transport agent to obtain current requirements.

Samples may also be hand delivered to the site during normal business hours (8.30am - 4.30pm).

Sample Type	Laboratory	Street Address
Animal (including aquatic animals)	NSW Animal and Plant Health Laboratories (APHL)	EMAI, Woodbridge Road, Menangle NSW 2568