Anthrax

Description

Anthrax is a zoonotic disease caused by the spore forming bacterium Bacillus anthracis. Although anthrax is common in herbivores (e.g. cattle, sheep, and goats) and wild animals, humans can be exposed to tissue from infected animals, or directly to B anthracis spores under certain conditions.  Most cases of anthrax in NSW occur in sheep and cattle within the geographical area known as the “anthrax belt”.

When anthrax is suspected, a post mortem should not be undertaken as it will cause the vegetative forms of Bacillus anthracis to sporulate. These spores will remain a source of infection for long periods.

Anthrax can have different clinical presentations depending on the route of infection, host factors, and strain-specific factors. Herbivores affected by anthrax commonly present with acute septicaemia with a high fatality rate, often accompanied by haemorrhagic lymphadenitis.

Signs of acute septicaemia include fever and hyperexcitability followed by depression, respiratory or cardiac distress, staggering, convulsions, and death. Animals can be found dead without signs of disease. There may be bloody discharges from the natural body orifices.

Diagnosis and tests available

Diagnosis

Diagnosis is strongly supported by history, clinical signs and the use of an Immunochromatographic test (ICT) in the field. Confirmation is by smear examination and PCR in the laboratory. If the ICT test is used, samples must still be submitted to the laboratory for confirmation.

ICT test kits can be ordered from the laboratory by contacting your Local Land Services District Vet.

Tests available

Click on + in the tables below for sampling requirements and additional test information

Code Name Samples required Species Turnaround time Price (excl. GST) Notes Test days
BA_ANT_PCR Anthrax PCR

Lith Hep (Green top)

  • 10ml of blood collected into a lithium heparin tube
  • Submit chilled

Fresh tissue/Ear Notch

  • A 2cm square portion of the ear (Bacillus anthracis DNA can be extracted from the tissue for PCR when no blood or fluid can be collected.)
  • Submit chilled

Clotted blood (Red top)

  • 10ml of blood collected into a plain red top tube
  • Submit chilled

EDTA blood (Purple top)

  • 10ml of blood collected into an EDTA tube
  • Submit chilled

Isolates grown in culture.

Refer to Anthrax Page for further information on how to send samples

Bovine, Ovine, Caprine, Porcine and Equine

1D

$154.20

D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.

2 Prior approval of delivery and testing on Saturday is required. Please contact Customer Service on 1800 675 623 to seek approval.

Monday – Saturday2

BA_ANT_SS Anthrax - microscopy

Blood smear

  • Blood smears from peripheral blood vessels (e.g. those in the ear)
    • Smears should be sent from more than one animal
    • Blood smears should be thin and air-dried, for Polychrome methylene blue (PMB) stain
    • Do not fix smears by heat or other agents
    • One end of the slide must be left clean

Fresh tissue

  • A 2cm square portion of the ear (Bacillus anthracis DNA can be extracted from the tissue for PCR when no blood or fluid can be collected.)
  • Submit chilled

Refer to Anthrax Page for further information on how to send samples

Bovine, Ovine, Caprine

1D

$34.25

D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.

2 Prior approval of delivery and testing on Saturday is required. Please contact Customer Service on 1800 675 623 to seek approval.

Monday – Saturday2

BANTH_QPCR Anthrax qPCR

Lith Hep (Green top)

  • 10ml of blood collected into a lithium heparin tube
  • Submit chilled

Fresh tissue/Ear Notch

  • A 2cm square portion of the ear (Bacillus anthracis DNA can be extracted from the tissue for PCR when no blood or fluid can be collected.)
  • Submit chilled

Clotted blood (Red top)

  • 10ml of blood collected into a plain red top tube
  • Submit chilled

EDTA blood (Purple top)

  • 10ml of blood collected into an EDTA tube
  • Submit chilled

Isolates grown in culture.

Refer to Anthrax Page for further information on how to send samples

Bovine, Ovine, Caprine, Porcine and Equine

1D

$154.20

D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.

2 Prior approval of delivery and testing on Saturday is required. Please contact Customer Service on 1800 675 623 to seek approval.

Monday – Saturday2

PLEASE NOTE

The preferred sample for anthrax testing is a peripheral blood smear. When an ICT test has been performed, please submit the used ICT kit in addition to any other samples. Where possible, please submit duplicate samples in case confirmatory testing is required.

Fees for tests undertaken to confirm or exclude a diagnosis of Anthrax are paid by NSW Department of Primary Industries and Regional Development (DPIRD).

Contact Customer Service to provide advance notice of your submission. This will allow specific biosecurity precautions to be undertaken at the laboratory.

Clearly label specimens as Suspect Anthrax. A Suspect Anthrax warning should also be placed under the lid of the outer package. This will ensure specific biosecurity precautions are undertaken at the laboratory.

Pack specimens securely (i.e. double bag and send in a rigid container) and forward to the laboratory separate from any other specimens.

Additional Information

Customer Service

Contact Customer Service for enquiries relating to testing and results, quotations and pricing, couriers, sample submission and invoicing.

NSW DPIRD's laboratories are committed to continual improvement of services. If you would like to provide feedback, please fill in and submit the online feedback form.

CONTACT DETAILS

Phone1800 675 623
Email

laboratory.services@dpird.nsw.gov.au

Operating Hours

8:30am-4:30pm Monday-Friday (excluding public holidays)

8:30am-12:00pm Saturday (for deliveries only)

Postal Address

Private Bag 4008, Narellan NSW 2567

Quotations

For Animal export, Plant health or Veterinary quotations, please complete the online quotation request

Submission Forms

Veterinary Specimen Advice Form

Please Note: These documents are not fully web accessible, please contact Customer Service (laboratory.services@dpird.nsw.gov.au) for more information.

Courier services and specimen delivery

For NSW submissions, please contact the relevant Customer Service team for courier account details and to order consignment notes.

For submitters outside of NSW, Couriers can be contacted directly to make bookings and arrange collection of packages at the submitters own cost.

Commercial couriers may use either road or air transport and specimens should therefore be packed in accordance with International Air Transport Association (IATA) requirements. Most submission sent by veterinarians to the APHL for testing are defined as “Biological Substance Category B” and must be packed according to the IATA packing instructions 650 (Biological Substance Category B). As requirements for transport can change, customers should consult with their transport agent to obtain current requirements.

Samples may also be hand delivered to the site during normal business hours (8.30am - 4.30pm).

Sample Type

Laboratory

Street Address

Animal (including aquatic animals)

NSW Animal and Plant Health Laboratories (APHL)

EMAI, Woodbridge Road, Menangle NSW 2568