Salmonellosis

Description

Salmonellosis is caused by many serotypes of Salmonella enterica subsp. enterica which are typically host specific. For example Salmonella enterica serovar Dublin (Salmonella Dublin) and Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) cause infection in cattle and sheep. Transmission is generally by the oral route. The bacteria colonize the gut, penetrate the intestinal wall and multiply in the cells of the monocyte-macrophage line in the spleen and liver. Salmonella then re-enter the gut from where they are shed.

Diagnosis and tests available

Diagnosis

Diagnosis is suggested by clinical signs, histopathology and necropsy findings and confirmed by bacterial culture.

Serum samples from cattle or sheep may be tested by the serum agglutination test (SAT) for Salmonella Dublin or Salmonella Typhimurium.

Tests available

Click on + in the tables below for sampling requirements and additional test information

Code	Name	Samples required	Species	Turnaround time	Price (excl. GST)	Notes	Test days
HIST_HE1	Histopathology – Preparation & Examination	Fixed Tissues- General Fresh tissues immediately placed in formalin Tissue pieces should not be greater than 1cm cubed to ensure proper fixation Submit fixed in neutral buffered formalin at a 10:1 ratio of formalin: tissue Fixed Tissues- Nervous tissues Submission of whole brain is required to ensure preservation of TSE target sites submit fixed in neutral buffered formalin at a ratio of 10:1 formalin: tissue Brains submitted for Nervous tissues are processed once a week, separate to general tissues to ensure consistent and reliable results. Urgent cases can be processed out of cycle if clearly marked on the Submission Form however this may reduce the quality of the brain histology results, so should only be requested in extremely urgent cases. Fresh Tissues Fresh tissues can be submitted following necropsy The quality of the tissues will be reduced due to the effects of autolysis so ideally fixed tissue should be submitted in neutral buffered formalin at a ratio of 10:1 formalin: tissue	All species	Up to 10D	$84.95	D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.	Monday - Friday
SA_DUB_SA	Salmonella dublin SAT	Clotted blood (Red top) 10 ml of blood collected from live animals into a plain red top tube. Submit chilled.	Bovine, Ovine	Up to 5D	$20.35	D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.	Wednesday & Thursday
SALM_EQPCR	Salmonella Enteriditis qPCR*	Faeces, environmental swabs, and eggs	Avian	2D	$109.35	* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.
SALM_GQPCR	Salmonella Gallinarum PCR*	Intestinal, cloacal content, cecal tonsil, liver, gall bladder, spleen and strains/vaccines	Avian	2D	$307.80	* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.
SA_GPD_ABE	Salmonella Group-D Antibody ELISA*	Clotted blood (Red top) 10 ml of blood collected from live animals into a plain red top tube. Submit chilled.	Avian	Up to 5D**	$29.05	* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. **1-2 weeks notice is required before submitting samples	Monday to Friday
SALM_MULTI	Salmonella Multiplex qPCR: Salmonella, Salmonella group D, S. Enteritidis*	Faeces, environmental swabs, and eggs	Avian	2D - Outbreak Response 5-7D - Surveillance	$137.80	* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.
SALM_PQPCR	Salmonella Pullorum PCR*	Intestinal, cloacal content, cecal tonsil, liver, gall bladder, spleen and strains/vaccines	Avian	2D	$307.80	* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.
SALM_DQPCR	Salmonella serogroup D qPCR*	Faeces, environmental swabs, and eggs	Avian	2D	$109.35	* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.
SALM_QPCR	Salmonella spp. qPCR*	Faeces, environmental swabs, and eggs	Avian	2D	$109.35	* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.
SALM_CLT	Salmonella – Selective culture	Fresh tissue Portions of liver, kidney, lung, bile, spleen, mesenteric lymph nodes and small intestinal contents collected from dead animals. Submit chilled. Faeces Faecal samples can be submitted from live animals. Submit chilled. MINIMUM of 20 grams required per animal (ruminants) for export of health testing. Environmental samples	All species	3-7D¹	$82.10	D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays. ¹4 - 7 working days for export, health testing or environmental samples	Monday to Saturday¹
SA_TYP_SA	Salmonella typhimurium SAT	Clotted blood (Red top) 10 ml of blood collected from live animals into a plain red top tube. Submit chilled.	Bovine, Ovine	Up to 5D	$20.35	D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.	Wednesday & Thursday
SALM_TQPCR	Salmonella Typhimurium PCR*	Faeces, environmental swabs, eggs	Bovine, Ovine	5D	$109.35	* Denotes analyses not covered by the NATA scope of accreditation. D (number of working days) and W (number of weeks). Test TAT does not include weekends and public holidays.

Additional specimen requirements

Fixed tissue for Histopathology
Sections of liver, kidney, lung, spleen, mesenteric lymph nodes, small and large intestine. Submit fixed in neutral buffered formalin at a 10:1 ratio of formalin: tissue.

Additional Information

Customer Service

Contact Customer Service for enquiries relating to testing and results, quotations and pricing, couriers, sample submission and invoicing.

NSW DPIRD's laboratories are committed to continual improvement of services. If you would like to provide feedback, please fill in and submit the online feedback form.

CONTACT DETAILS
Phone	1800 675 623
Email	laboratory.services@dpird.nsw.gov.au
Operating Hours	8:30am-4:30pm Monday-Friday (excluding public holidays) 8:30am-12:00pm Saturday (for deliveries only)
Postal Address	Private Bag 4008, Narellan NSW 2567

Quotations

For Animal export, Plant health or Veterinary quotations, please complete the online quotation request

Submission Forms

Veterinary Specimen Advice Form

Please Note: These documents are not fully web accessible, please contact Customer Service (laboratory.services@dpird.nsw.gov.au) for more information.

Courier services and specimen delivery

For NSW submissions, please contact the relevant Customer Service team for courier account details and to order consignment notes.

For submitters outside of NSW, Couriers can be contacted directly to make bookings and arrange collection of packages at the submitters own cost.

Commercial couriers may use either road or air transport and specimens should therefore be packed in accordance with International Air Transport Association (IATA) requirements. Most submission sent by veterinarians to the APHL for testing are defined as “Biological Substance Category B” and must be packed according to the IATA packing instructions 650 (Biological Substance Category B). As requirements for transport can change, customers should consult with their transport agent to obtain current requirements.

Samples may also be hand delivered to the site during normal business hours (8.30am - 4.30pm).

Sample Type	Laboratory	Street Address
Animal (including aquatic animals)	NSW Animal and Plant Health Laboratories (APHL)	EMAI, Woodbridge Road, Menangle NSW 2568